Volume 8 - Issue 2


Article Number 1
Title

Albomagister virgineus sp. nov., a second species of Albomagister in Europe

Authors

Corriol G and Jargeat P

Received 25 August 2017
Accepted 26 December 2017
Published Online 16 February 2018
Corresponding Author Gilles Corriol – e-mail – gilles.corriol@cbnpmp.fr
Abstract

Albomagister virgineus is described as a new species from a collection from Hautes-Pyrénées in France. Morphological and molecular studies clearly separate it from related species of Albomagister. The assignment to Albomagister is presently suitable, however, morphological characters and a complementary morphological analysis of A. alesandrii, the only representative of the genus in Europe, contradicted the recent circumscription of this genus. Molecular results confirmed these findings and suggest heterogeneity among the Albomagister.

Keywords 1 new species – Basidiomycota – France – Hautes-Pyrénées– Taxonomy – Tricholomataceae
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Article Number 2
Title

Mycodiversity and biotechnological potential of endophytic fungi isolated from hydrophytes

Authors

Rajagopal K, Meenashree B, Binika D, Joshila D, Tulsi PS, Arulmathi R, Kathiravan G, Tuwar A

Received 02 November 2017
Accepted 16 December 2017
Published Online 16 February 2018
Corresponding Author Meenashree B. – e-mail – menugenes@gmail.com
Abstract

Foliar endophytic fungi were isolated from regular hydrophytic plants such as Eichhornia crassipes (Pontederiaceae), Nymphaea nouchali (Nymphaeaceae), Vallisneria spiralis (Hydrocharitaceae) growing in a pond in the Anna Zoological Park, Chennai, Tamil Nadu. Five hundred leaf segments from each plant species were inoculated in Potato Dextrose Agar (PDA) medium. A total of 18 different endophytic fungi could be isolated from three host surveyed. Hyphomycetes group was the most prevalent endophyte than ascomycetes, coelomycetes, and sterile form. Nymphaea nouchali had more endophytic fungi (18 species) followed by Eichhornia crassipes (12 species), and Vallisneria spiralis (11 species). To our knowledge Vallisneria spiralis is studied for endophytic fungi diversity for the first time. Among the 18 endophytic fungi isolated, nine were present in all three hosts investigated for endophytic fungi. Curvularia lunata, Chaetomium indicum, Nigrospora oryzae, Pestalotiopsis sp. showed <5% of colonization frequency in all three host species studied. The culture filtrate of Curvularia lunata, Nigrospora oryzae, Chaetomium indicum and Pestalotiopsis microspora investigated contained alkaloids, flavonoids, terpenoids, tannins and steroids. The dominant endophytic fungi were tested for production of extracellular enzymes like amylase, cellulase, L- asparaginase, laccase and protease. Curvularia lunata, Chaetomium indicum and Pestalotiopsis microspora produced all the enzymes tested, whereas Nigrospora oryzae did not produce L- asparaginase. The culture filtrate of Chaetomium indicum and Pestalotiopsis microspora significantly increased the cell division in Allium cepa root meristem and the radical plumule length in AD8 rice variety.

Keywords Eichhornia crassipes – Nymphaea nouchali – Vallisneria spiralis – Hydrophyte – Leaves – Bioactive compounds
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Article Number 3
Title

Tracing an inoculated arbuscular mycorrhizal fungus, Funneliformis mosseae, in a field experiment using molecular tools

Authors

Thilagar G, Anshu BR, Bagyaraj DJ, Mathimaran N, Jawali N

Received 22 November 2017
Accepted 30 January 2018
Published Online 16 February 2018
Corresponding Author D J Bagyaraj – e-mail – djbagyaraj@gmail.com
Abstract

The aim of this study was to assess the presence of inoculated AM fungus Funneliformis mosseae (earlier called Glomus mosseae), applied as part of the microbial consortia (MC) in the root zone of chilly (Capsicum annuum L.) plants from the field experiment through nested PCR based approach. A field experiment was conducted with 100% recommended level of chemical fertilizers and 50% recommended level of chemical fertilizers plus selected microbial consortia i.e., Funneliformis mosseae + Bacillus sonorensis in order to find out the possibility of reducing the recommended level of chemical fertilizer for cultivation of chilly. The introduced inoculum Funneliformis mosseae was tracked from the field by amplifying a region of rRNA gene using specific primers followed by sequencing. Total DNA was extracted from the roots of chilly plants. The universal eukaryote primer pair NS5/ITS4 was initially used for the first amplification and further amplified by Glomeraceae specific primer GLOM1310, in conjugation with the universal primer ITS4i. The amplicon was obtained only from plant root inoculated with microbial consortia and its sequence validated the presence of F. mosseae.

Keywords AM fungus – Chilly – Nested PCR
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About CREAM Journal

Current Research in Environmental & Applied Mycology publishes reviews, research articles, methodology papers, taxonomic works such as monographs, and checklists which are relevant to fungal biology, including lichens. The official journal language is English.

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